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Gene-specific 5′ RACE products appear as a smear. Inhibitors of RT present Remove inhibitors by ethanol precipitation of the mRNA preparation before the first strand reaction. Band intensity and size distribution of resulting products depends on the specificity of GSPs used for cDNA synthesis and PCR, the complexity and relative abundance of target cDNA, and the PCR conditions used. Control RNA, DNA, and primers are included to verify the performance of the system and may be added to experimental RNA preparations to monitor the efficiency of each step or to troubleshoot potential problems.

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